This document contains specifications for active sampling of bioaerosols from exhaust air flowing through a defined cross-section of a stack. It defines general principles that have to be taken into account during an isokinetic sampling campaign for bioaerosols by bubbling the exhaust air through a specific impinger designed for emission measurements.

In this document the application with culturable organisms is specified but the same principle might be applicable for non-cultural based methods (e.g. molecular and/or enzyme-based methods).

The impinger is designed to allow a sample volume flow of 1 m³/h to 1,8 m³/h, or 16 l/min to 30 l/min, respectively, and has been tested with regard to various microorganisms within broad concentration ranges [1; 2; 3; 4]. ¹