Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae - Detection of Enterobacteriaceae

Microbiology of the food chain. Horizontal method for the detection and enumeration of Enterobacteriaceae - Detection of Enterobacteriaceae

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1   Scope

This document specifies a method, with enrichment, for the detection of Enterobacteriaceae. It is applicable to
  • products intended for human consumption and the feeding of animals, and
  • environmental samples in the area of primary production, food production and food handling.
This method is applicable
  • when the microorganisms sought are expected to need resuscitation by enrichment, and
  • when the number sought is expected to be below 100 per millilitre or per gram of test sample.
A limitation on the applicability of this document is imposed by the susceptibility of the method to a large degree of variability (see Clause 11).

NOTE Enumeration can be carried out by calculation of the most probable number (MPN) after incubation in liquid medium. See Annex A.