1 Scope
This part of ISO 21528 specifies a method, without pre-enrichment, for the enumeration of Enterobacteriaceae. It is applicable to
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— products intended for human consumption and the feeding of animals, and
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— environmental samples in the area of food production and food handling.
Enumeration is carried out by counting colonies in a solid medium after incubation at 37 °C (or 30 °C)1).
This technique is recommended when the number of colonies sought is expected to be more than 100 per millilitre or per gram of the test sample.