Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli - Colony-count technique at 44°C using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide

Microbiology of food and animal feeding stuffs. Horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli - Colony-count technique at 44°C using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide

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1   Scope

This part of ISO 16649 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli in products intended for human consumption or for the feeding of animals. It uses a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase.

WARNING — Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are β-glucuronidase negative, such as Escherichia coli O157, will not be detected.