Infant formula and adult nutritionals. Simultaneous determination of total vitamins B<sub>1</sub>, B<sub>2</sub>, B<sub>3</sub> and B<sub>6</sub>. Enzymatic digestion and LC-MS/MS

Infant formula and adult nutritionals. Simultaneous determination of total vitamins B1, B2, B3 and B6. Enzymatic digestion and LC-MS/MS

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1   Scope

This document specifies a method for the simultaneous quantitative determination of four water-soluble vitamins in infant formula and related nutritional products, including relevant forms of vitamins B1, B2, B3 and B6 by enzymatic digestion and UHPLC-MS/MS. This document is not intended to be used on products where vitamins have not been added.