Foodstuffs. Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection

BS EN 15850:2010 Foodstuffs. Determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection

BS EN 15850 specifies a method for the determination of zearalenone in maize based baby food, barley flour, maize flour, polenta, wheat flour and cereal based foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection.

This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of maize based baby food, barley flour, maize flour, polenta and wheat flour ranging from 10 μg/kg to 335 μg/kg, and the second study was for samples of cereal based foods for infants and young children ranging from 9 μg/kg to 44 μg/kg.

Further information on validation, see Clause 9 and Annex B.

Contents of BS EN 15850:

Foreword

1 Scope

2 Normative references

3 Principle

4 Reagents

5 Apparatus

6 Procedure

7 HPLC analysis

8 Calculation

9 Precision

10 Test report

Annex A (informative) Typical chromatograms

Annex B (informative) Precision data

Bibliography